marker isolectin b4  (Vector Laboratories)

Journal: Basic Research in Cardiology

Article Title: Effects of sex and obesity on immune checkpoint inhibition-related cardiac systolic dysfunction in aged mice

doi: 10.1007/s00395-024-01088-4

Figure Lengend Snippet: Histological analyses of the hearts. a Cell surface area is significantly increased in the male HFD + L-NAME vs. CON diet groups, nevertheless, hypertrophy is present in CON-fed females, and is not further enhanced by HFD + L-NAME. b Representative images for cell surface area and microvascular density analyses. c Microvascular density is significantly decreased in the male HFD + L-NAME vs. CON diet groups, nevertheless, smaller microvascular density is present in CON-fed females, and is not further decreased by HFD + L-NAME. d Fibrotic area is significantly increased in the male HFD + L-NAME vs. CON diet groups, nevertheless, no change in females is achieved. e Representative images for fibrotic area analyses. Each data point represents values derived from individual experimental animals. For statistical analyses, two-way ANOVA with Sidak’s post-hoc test were used. All values are presented as mean ± standard deviation. *: P < 0.05 vs. corresponding control. CON control diet ( n = 6 for males and n = 6 for females), HFD + L-NAME high fat diet plus L-NAME (HFD + L-NAME, n = 7 for males and n = 5 for females), WGA wheat germ agglutinin, ILB4 isolectin B4

Article Snippet: To assess cardiomyocyte hypertrophy and microvascular density, cardiac sections underwent antigen retrieval (citrate buffer pH 6, Vector Laboratories, Newark, CA, USA, H-3300) for 30 min, then incubated with wheat germ agglutinin (WGA–FITC—marker of cell membrane, 1:50, Sigma Aldrich, L4895) and with isolectin B4 (ILB4-DyLight 594—marker of cardiac endothelial cells, 1:50, Invitrogen, L32473) overnight at 4 °C.

Techniques: Derivative Assay, Standard Deviation, Control

Journal: Basic Research in Cardiology

Article Title: Effects of sex and obesity on immune checkpoint inhibition-related cardiac systolic dysfunction in aged mice

doi: 10.1007/s00395-024-01088-4

Figure Lengend Snippet: Western blot analyses of spleens. a Representative western blot pictures for all analyzed proteins. b Splenic protein expression of Tox/Tox2 in males and females. c Splenic protein expression of Tcf1/7 in males and females. d Splenic protein expression of PD-1 in males and females. e Splenic protein expression of TIM3 in males and females. f Splenic protein expression of VISTA in males and females. g Splenic protein expression of OX40 in males and females. For statistical analyses, Student’s unpaired t -test was used. All values are presented as mean ± standard deviation. *: P < 0.05 vs. corresponding control. CON control diet ( n = 5 for males and n = 5 for females), HFD + L-NAME high fat diet plus L-NAME (HFD + L-NAME, n = 6 for males and n = 6 for females), WGA wheat germ agglutinin, ILB4 isolectin B4

Article Snippet: To assess cardiomyocyte hypertrophy and microvascular density, cardiac sections underwent antigen retrieval (citrate buffer pH 6, Vector Laboratories, Newark, CA, USA, H-3300) for 30 min, then incubated with wheat germ agglutinin (WGA–FITC—marker of cell membrane, 1:50, Sigma Aldrich, L4895) and with isolectin B4 (ILB4-DyLight 594—marker of cardiac endothelial cells, 1:50, Invitrogen, L32473) overnight at 4 °C.

Techniques: Western Blot, Expressing, Standard Deviation, Control

Journal: Basic Research in Cardiology

Article Title: Effects of sex and obesity on immune checkpoint inhibition-related cardiac systolic dysfunction in aged mice

doi: 10.1007/s00395-024-01088-4

Figure Lengend Snippet: Histological analyses of the hearts of mice treated with immune checkpoint inhibitor. a Cell surface area is significantly increased in the male HFD + L-NAME vs. CON diet groups, nevertheless, hypertrophy is present in CON-fed females, and is not further enhanced by HFD + L-NAME. b Representative images for cell surface area and microvascular density analyses. c Microvascular density is significantly decreased in the male HFD + L-NAME vs. CON diet groups, nevertheless, smaller microvascular density is present in CON-fed females, and is not further decreased by HFD + L-NAME. d Fibrotic area does not change in any of the groups irrespective of sex or diet. e Representative images for fibrotic area analyses. Each data point represents values derived from individual experimental animals. For statistical analyses, two-way ANOVA with Sidak’s post-hoc test were used. All values are presented as mean ± standard deviation. *: P < 0.05 vs. corresponding control. CON control diet ( n = 6 for males and n = 6 for females), HFD + L-NAME high fat diet plus L-NAME (HFD + L-NAME, n = 6 for males and n = 7 for females), WGA wheat germ agglutinin, ILB4 isolectin B4

Article Snippet: To assess cardiomyocyte hypertrophy and microvascular density, cardiac sections underwent antigen retrieval (citrate buffer pH 6, Vector Laboratories, Newark, CA, USA, H-3300) for 30 min, then incubated with wheat germ agglutinin (WGA–FITC—marker of cell membrane, 1:50, Sigma Aldrich, L4895) and with isolectin B4 (ILB4-DyLight 594—marker of cardiac endothelial cells, 1:50, Invitrogen, L32473) overnight at 4 °C.

Techniques: Derivative Assay, Standard Deviation, Control

Journal: Basic Research in Cardiology

Article Title: Effects of sex and obesity on immune checkpoint inhibition-related cardiac systolic dysfunction in aged mice

doi: 10.1007/s00395-024-01088-4

Figure Lengend Snippet: Western blot analyses of spleens of mice treated with immune checkpoint inhibitor. a Representative western blot pictures for all analyzed proteins. b Splenic protein expression of Tox/Tox2 in males and females. c Splenic protein expression of Tcf1/7 in males and females. d Splenic protein expression of PD-1 in males and females. e Splenic protein expression of TIM3 in males and females. f Splenic protein expression of VISTA in males and females. g Splenic protein expression of OX40 in males and females. For statistical analyses, Student’s unpaired t -test was used. All values are presented as mean ± standard deviation. *: P < 0.05 vs. corresponding control. CON control diet ( n = 6 for males and n = 6 for females), HFD + L-NAME high fat diet plus L-NAME (HFD + L-NAME, n = 6 for males and n = 6 for females), WGA wheat germ agglutinin, ILB4 isolectin B4

Article Snippet: To assess cardiomyocyte hypertrophy and microvascular density, cardiac sections underwent antigen retrieval (citrate buffer pH 6, Vector Laboratories, Newark, CA, USA, H-3300) for 30 min, then incubated with wheat germ agglutinin (WGA–FITC—marker of cell membrane, 1:50, Sigma Aldrich, L4895) and with isolectin B4 (ILB4-DyLight 594—marker of cardiac endothelial cells, 1:50, Invitrogen, L32473) overnight at 4 °C.

Techniques: Western Blot, Expressing, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Central Connectivity of Transient Receptor Potential Melastatin 8-Expressing Axons in the Brain Stem and Spinal Dorsal Horn

doi: 10.1371/journal.pone.0094080

Figure Lengend Snippet: (A–D) Double-immunofluorescence staining for Trpm8 GFP and markers for peptidergic C nociceptive neurons, CGRP (A) and SP (B), and for non-peptidergic C nociceptive neurons, IB4 (C), and P2X 3 (D). colocalization is represented in white in the merged images (arrowheads). (E–H) Quantitative analysis of the co-staining of Trpm8 GFP with CGRP (E), SP (F), IB4 (G), and P2X 3 (H). Of all TRPM8+ neurons, 26.2% (305/1164 in 12 sections) co-stain for CGRP, 24.3% (207/853 in 11 sections) co-stain for SP, 1.3% co-stain for IB4 (4/308 in 10 sections), and 1.2% co-stain for P2X 3 (6/486 in 10 sections). Scale bars = 50 μm.

Article Snippet: For immunofluorescent staining for the non-peptidergic marker Griffonia simplicifolia isolectin B4 (IB4), sections were preincubated with 1 μg/ml IB4 (L-1104; Vector Laboratories; Burlingame, CA, USA) for 16 hours and then reacted with rabbit anti-GFP and goat anti-IB4 (1∶3,000; AS2104; Vector Laboratories) antibodies overnight.

Techniques: Double Immunofluorescence Staining, Staining